Enzyme-linked immunosorbent assay (ELISA) experimental test services - Database & Sql Blog Articles

**Enzyme-Linked Immunosorbent Assay (ELISA)** **Overview of ELISA Testing** ELISA, or Enzyme-Linked Immunosorbent Assay, is a widely used and highly sensitive technique in immunology that combines the specificity of antigen-antibody interactions with the catalytic efficiency of enzymes. This method allows for the detection and quantification of various substances, such as proteins, antibodies, and hormones, by utilizing a solid-phase support—typically a polystyrene microtiter plate. During the process, each reagent is added sequentially, and excess unbound components are removed through washing, ensuring high specificity and reproducibility. The final color change, which results from the enzymatic reaction on a substrate, is measured using a spectrophotometer to determine the concentration of the target molecule. **Common ELISA Methods** There are several variations of ELISA depending on the type of molecule being tested. These include: - **Indirect ELISA:** Used to detect antibodies. - **Sandwich ELISA:** Ideal for detecting antigens, where two antibodies are used—one to capture the antigen and another to detect it. - **Competition ELISA:** Useful for small molecules or haptens, where the presence of the target molecule competes with a labeled version for binding sites. At Jinma Life Science Research Center, we commonly offer both the **sandwich ELISA** and **indirect ELISA** methods based on our clients' needs. **Experimental Technical Process** 1. **Coating**: The antigen or antibody is immobilized onto the surface of a microtiter plate. 2. **Incubation with Sample**: The sample is added, allowing any specific antibodies or antigens present to bind. 3. **Addition of Enzyme-Conjugated Antibody**: This step helps detect the bound complex. 4. **Substrate Addition**: A substrate is introduced, which reacts with the enzyme to produce a measurable color change. 5. **Stop Solution**: Added to halt the reaction and stabilize the color. 6. **Analysis**: The intensity of the color is measured and correlated with the concentration of the target molecule. **Service Process** 1. **Pre-Sales Support**: Our technical team provides guidance on specimen collection and experimental design. 2. **Sample Collection**: Clients collect and send their samples to us. Shanghai-based clients may arrange for pickup. 3. **Testing**: Our laboratory conducts the ELISA testing according to the protocol. 4. **Result Delivery**: After payment, the results are sent to the client via the sales representative. 5. **Post-Sales Support**: If you have any questions, contact your sales representative, who will coordinate with our technical team for further assistance. **Customer Notice** - **Specimen Types**: Serum, plasma, urine, cell culture supernatants, tissue homogenates, cerebrospinal fluid, pleural effusion, ascites, etc. - **Sample Volume**: Each sample should be collected in a volume of (20–50 µL) multiplied by the number of detection indicators. - **Storage**: For long-term storage, samples must be kept at -20°C or -70°C. If collecting in batches, store them immediately after collection. - **Preparation**: Please inform us about the species (human, rat, mouse, rabbit, pig, etc.), number of samples, and the specific markers to be tested. - **Labeling**: When sending samples, please include: - Specimen number - Tested item - Whether duplicates are needed - Contact information - Whether the sample should be returned after testing - **Transportation**: Use SF Express or Zhongtong Express. Store samples in an insulated box with dry ice or ice packs to maintain low temperature. **Report Content and Standards** - ELISA standard curve - Detailed experimental procedure - Full experimental report including instruments used, results, and interpretation **Charging Standards and Service Cycle** The cost and service timeline depend on the sample type and complexity. For specific details, please contact our customer service representative or refer to the signed cooperation agreement. **Quality Assurance** Our ELISA tests are suitable for a wide range of biological specimens, offering high sensitivity and accuracy. While we ensure reliable and objective results, we cannot guarantee specific outcomes due to variability in biological samples. **After-Sales Service** We conduct regular follow-ups with our clients to improve our service quality. The terms of after-sales support are outlined in the signed cooperation contract and may vary accordingly. --- The following data represents the detection results for **Human S100 Calcium Binding Protein A8/A9 Complex (S100A8/A9)** provided by Jinma Life Science Research Center. *(For reference only; actual test results may vary based on the specific conditions of the experiment)*

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