Enzyme-linked immunosorbent assay (ELISA) experimental test services - Database & Sql Blog Articles

**Enzyme-Linked Immunosorbent Assay (ELISA)** **1. Overview of ELISA Testing** ELISA, or Enzyme-Linked Immunosorbent Assay, is a widely used and highly sensitive laboratory technique that combines the specificity of antigen-antibody interactions with the catalytic efficiency of enzymes. This method allows for the detection and quantification of various substances, such as proteins, antibodies, and hormones, in biological samples. The process takes place on a solid-phase carrier, typically a polystyrene microtiter plate. After each reagent addition, excess unbound material is washed away, ensuring high specificity and accuracy in the final results. ELISA is known for its reliability, sensitivity, and ease of use, making it a popular choice in both research and diagnostic settings. **2. Common ELISA Methods** There are several variations of ELISA, each suited to different testing needs: - **Indirect ELISA**: Used to detect antibodies in a sample by using a labeled secondary antibody. - **Sandwich ELISA**: Ideal for detecting antigens, where two antibodies (capture and detection) bind to the target antigen. - **Competitive ELISA**: Often used for small molecules or haptens, where the presence of the analyte competes with a labeled version for binding sites. We currently offer services based on the **double-antibody sandwich method** and the **indirect ELISA**, which are among the most commonly used techniques in our lab. **3. Experimental Technical Process** Here’s a general workflow for an ELISA experiment: 1. **Coating**: The antigen or antibody is immobilized onto the surface of a microplate well. 2. **Incubation with Sample**: The test sample is added, allowing the target molecule to bind to the coated antigen or antibody. 3. **Addition of Enzyme-Labeled Reagent**: An enzyme-conjugated secondary antibody or antigen is introduced to bind to the complex. 4. **Substrate Addition**: A substrate is added, which reacts with the enzyme to produce a measurable color change. 5. **Stop Solution**: The reaction is stopped to prevent overdevelopment. 6. **Analysis**: The intensity of the color is measured using a spectrophotometer, allowing for both qualitative and quantitative analysis. **4. Service Process** Our service includes the following steps: 1. **Pre-Sales Support**: Our technical team is available to provide guidance on sample collection, storage, and preparation. 2. **Sample Collection and Delivery**: Customers can collect their samples and send them to us. In Shanghai, we also offer pickup options. 3. **Testing**: Our lab conducts the ELISA tests according to your requirements. 4. **Result Delivery**: Once the payment is processed, the test results are sent to you via your assigned sales representative. 5. **Follow-Up**: If you have any questions about the results, contact your sales representative, who will coordinate with the technical team for further support. **5. Customer Notice** To ensure accurate and reliable results, please note the following: - **Sample Types**: Include serum, plasma, urine, cell culture supernatants, tissue homogenates, and more. - **Sample Volume**: Each sample should be collected in a volume of (20–50 µL) multiplied by the number of detection indicators. - **Storage**: For long-term storage, samples must be frozen at –20°C or –70°C. If collecting in batches, store immediately after collection. - **Information Required**: Please specify the species (human, rat, mouse, etc.), the number of samples, and the specific detection targets. - **Sample Labeling**: When sending samples, include: - Specimen number - Tested item - Whether duplicates are required - Contact information - Whether the sample will be returned after testing - **Shipping**: Use express delivery services like SF Express or ZTO. Keep samples cool during transport using dry ice or ice packs inside an insulated box. **6. Report Content** Each report includes: - A detailed ELISA standard curve - Step-by-step experimental procedures - Complete experimental data, including instruments used, results, and interpretations **7. Pricing and Timeline** Pricing and service duration depend on the type and volume of samples provided. For specific details, please consult our customer service team or refer to your signed cooperation agreement. **8. Quality Assurance** Our ELISA tests are designed for high sensitivity and accuracy, suitable for a wide range of biological samples. While we guarantee the reliability of our results, we cannot guarantee specific outcomes due to variability in biological systems. **9. After-Sales Support** We conduct regular follow-ups to improve our service quality. Details of after-sales support are outlined in the cooperation contract, and may vary depending on the agreement. --- Below are the detection data for **Human S100 Calcium-Binding Protein A8/A9 Complex (S100A8/A9)** provided by the **Jinma Life Science Research Center**. *Note: This is for reference only. Actual test results may vary based on specific conditions.*

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